Michael Tessler and Christopher Mason on 16S amplicon vs shotgun sequencing (#9)
August 18, 2017
Michael Tessler and Christopher Mason join me to talk about their comparison of 16S amplicon sequencing and shotgun sequencing for quantifying microbial diversity.
Links:
- The 2017 Nature paper that we discuss: Large-scale differences in microbial biodiversity discovery between 16S amplicon and shotgun sequencing
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Michael’s et al. 2016 paper that describes their original 16S study: A Global eDNA Comparison of Freshwater Bacterioplankton Assemblages Focusing on Large-River Floodplain Lakes of Brazil
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The sequencing data for these studies is available from NCBI: PRJNA310230 (16S), PRJNA389803 (shotgun)
- Michael’s website
- The Integrative Biology & Medicine conference, featuring the talk on Postmodern Synthesis by Eugene Koonin
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Music: Eric Skiff — Come and Find Me (modified, licensed under CC BY 4.0).